中华皮肤科杂志 ›› 1993, Vol. 26 ›› Issue (3): 159-162.

• 论著 • 上一篇    下一篇

免疫斑点试验改良法检测麻风杆菌特异性抗原

曹元华1, 和泉真藏2, 畑野研太郎3, Mauro F. Mendes4   

  1. 1. 中国医学科学院皮肤病研究所, 南京, 210442;
    2. 日本厚生省国立多摩研究所;
    3. 孟加拉国基督教麻风中心;
    4. 巴西Socicdade Filantropical Humanitas
  • 收稿日期:1992-04-28 修回日期:1992-06-29 出版日期:1993-06-15 发布日期:1993-06-15

Detectian of PGL-I antigen froth M. Leprae by modified Dot-ELISA

CAO Yuan-Hua1   

  1. Institute of Dtrmatology, Chinese Academy of Medical Sciences, Nanjing 210042
  • Received:1992-04-28 Revised:1992-06-29 Online:1993-06-15 Published:1993-06-15

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摘要： 我们用免疫斑点试验改良法(M-Dot-ELISA)检测麻风杆菌特异性酚糖脂(PGL-I)抗原,并与检测特异性抗体的两种方法进行了对比.M-Dot-ELISA的特异性为97.5%.敏感性为35.8%.在LL和BL型麻风其敏感性可分别达95%.75%.麻风患者血清中PGL-I的量与抗体的OD值之间呈非常显著的正相关(r=0.993,Z>2,58,P<0.01).本改良法不需特殊仪器,可肉眼到断结果,具有简单、快速、特异,并可对抗原进行定量等优点.

关键词: 麻风血清诊断

Abstract: We developed a modified Dot-ELISA (M-Dot-ELISA) for detecting phenolic glycolipid I (PGL-I) antigen fmm M. leprae and compared with microplate ELISA (MicELISA) and Mycobacterium leprae particle agglutination (MLPA) test. The sensitivity of M-Dot-ELISA in leprosy patients is 35.8%, but in LL and BL patients is 95% and 75% respectively. The specificity of M-Dot-ELISA is 97.5%. The quantity of pGL-I correlated with the OD value of IgM antibody (r=0.993, z=2.58, P<0.01) in leprosy patients. M-Dot-ELISA does not need special equipments, and is simple, rapid, specific and economic, the results can be read out macroscopically andis easy to carry out under field condition for the dLtection of M. leprae infection. The method has not been reported in our counkry so far.

Key words: Leprosy, Serodiagnosis