中华皮肤科杂志 ›› 1993, Vol. 26 ›› Issue (3): 145-146.

• 论著 • 上一篇    下一篇

淋球菌耐药质粒的提取和限制性酶切分析

周莹1, 叶顺章2   

  1. 1. 现在中国预防医学科学院病毒学研究所, 北京, 100052;
    2. 中国医学科学院皮肤病研究所
  • 收稿日期:1992-08-25 修回日期:1992-11-23 出版日期:1993-06-15 发布日期:1993-06-15

Extractson and analysis of plasmid in penicillinase-producing Neisseria gooorrnoeae

ZHOU Ying1, YE Shun-Zhang2   

  1. Irutatute of Dermatology, Chinese Acadamy of Medicat Scaences, Nanjang 210042
  • Received:1992-08-25 Revised:1992-11-23 Online:1993-06-15 Published:1993-06-15

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摘要： 我们从300株淋球菌临床分离株中筛选出产青霉素酶淋球菌菌株(PPNG),进行了质较提取,回收了可能编码β内酞胺酶的5.4kb质粒,进行了限制性核酸内切酶的部分酶切切点分析.结果表明5.4kh的质粒很可能为所筛选出的PPNG菌株的耐药质粒.我们建立了成本较低的质粒提取方法,为流行病学的研究和质粒探针的制备打下了基础.

关键词: 淋病奈瑟菌, 药物耐受性, 质粒

Abstract: This paper reports a study of the PPNG strains isolated from the city of Nanjing. A total of 300 clinical specimens were collected for screening the PPNG strains. The incidence of PPNG strains was 3.3%. Plasmids of 10 PPNG and 30 non-PPNG strains were extracted and identified by a simple agarose gel electrophoretic method. The molecular weight of plasmid DNA was estimated by companng its migration in gel with DNA preparation of known molecular weights(λ DNA/HindⅢ digest marker). In either PPNG or non-PPNG strains, the most frequent plasmid profile was a combination of 4.2kb, 5.4kb and 39.5kb plasmids. The 5.4kb DNA band was reextracted from agarose gel and digested by restriction endonuclease. The result of the digest pattern suggests that 5.4kb plasmid may be the resistant plasmid encoding for β-lactamase of PPNG in Nanjing.

Key words: Neisseria, gonorrhoeae Drug resistance, Plasmids