中华皮肤科杂志 ›› 1991, Vol. 24 ›› Issue (2): 78-80.

• 论著 • 上一篇    下一篇

银屑病表皮基底细胞中钙调素含量测定

周京1, 王光超1, 李素文2, 薛绍白2   

  1. 1. 北京医科大学第一医院皮肤科;
    2. 北京师范大学生物系
  • 收稿日期:1989-08-14 修回日期:1989-12-14 出版日期:1991-04-15 发布日期:1991-04-15

Study of calmodulin in dispersed psoriatic basal cells

ZHOU Jing1, WANG Guang-Chao1   

  1. The First Hopital of Beijing Medical University
  • Received:1989-08-14 Revised:1989-12-14 Online:1991-04-15 Published:1991-04-15

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摘要： 采用细胞分光光度术对银屑病病人皮损区和非皮损区的单个表皮墓底细胞中钙调素(CaM)进行了测定.发现皮损区总CaM和活化型CaM均高于正常人,非皮损区总CaM高于正常人而活化型CaM与正常人无明显差别.通过复染DNA,可见随着细胞的G1-S-G2M周期的变化,CaM呈递增的趋势.

Abstract: Measurement of calrnoduliu (CaM) was made with microspectrophotometer in psoriatic dispersed epidermal basal cells. Fluorescence from total CaM was induced by anti-CaM-FITC and that from activated CaM was induced by its antaguoist trifluoperazine(TFP). The results showed that both CaM and active. ted CaM were siginificantly higher in psoriatic basal oelis (10 patients) than normal controls.Level of totxl CaM in the uninvolved basal cells was also elevated but to a lesser degree. Sut the activated CaM in uninvolved skin showed no significant difference with normal control. Meanwhile, total CaM and activated CaM in both normal and diseased cells were increased from G1 through S to C2M.