中华皮肤科杂志

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尖锐湿疣组织中人乳头状瘤病毒感染与生存素和增殖细胞核抗原的表达

林云, 涂亚庭, 陈善娟, 陈思远, 张丽霞, 吴艳, 陶娟, 陈金波   

  1. 华中科技大学同济医学院附属协和医院皮肤科 武汉 430022
  • 收稿日期:2005-06-10 出版日期:2006-04-15 发布日期:2006-04-15

HPV infection and its correlation with survivin and PCNA expression in condyloma acuminatum

LIN Yun, TU Ya-ting, CHEN Shah-juan, CHEN Si-yuan, ZHANG Li-xia, WU Yan, TAO Juan, CHEN Jin-bo   

  1. Department of Dermatology, The Affiliated Xiehe Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • Received:2005-06-10 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 探讨人乳头状瘤病毒(HPV)6/11、16/18在尖锐湿疣(CA)组织中的感染情况及其与生存素和增殖细胞核抗原(PCNA)表达的关系。方法 HPV分型PCR检测试剂盒检测HPV6/11、16/18在41例尖锐湿疣组织、23例正常皮肤组织中的表达情况,同时用SP免疫组化法检测生存素及PCNA蛋白的表达。结果 ①正常对照组未检测到HPV6/11、16/18感染;HPV6/11在尖锐湿疣中的感染率为87.80%(36/41),与正常对照组比较差异有统计学意义(P<0.005);HPV16/18在尖锐湿疣中的感染率为19.51%(8/41)广与正常对照组比较差异无统计学意义(P>0.05)。②生存素和PCNA在尖锐湿疣组织中的阳性率分别为53.66%(22/41)和87.80%(36/41),在正常皮肤组织中的阳性率分别为8.70%(2/23)和47.83%(11/23)。尖锐湿疣组中生存素和PCNA表达的阳性率与正常对照组比较差异均有统计学意义,且尖锐湿疣中两者的阳性表达呈正相关(P=0.009)。③生存素及PCNA在有HPV6/11感染患者中的表达明显强于无HPV6/11感染患者。结论 HPV感染可上调生存素与PCNA的表达。

关键词: 增殖细胞核抗原, 生存素, 尖锐湿疣, 乳头状瘤病毒, 人

Abstract: Objective To investigate HPV infection and its correlation with the expression of survivin and proliferating cell nuclear antigen (PCNA) in condyloma acuminatum (CA).Methods HPV6/11 and 16/18 DNA were detected by PCR in 41 cases of CA and 23 specimens of normal skin.The expression of survivin and PCNA was measured by immunohistochemical technique in these two groups.Results The infection rates of HPV6/11 and 16/18 were 87.80% (36/41) and 19.51%(8/41),respectively,in CA,and 0 in normal skin epidermis.The expression of survivin and PCNA was found in 53.66% (22/41) and 87.80% (36/41) of CA ,and was 8.70%(2/23)and 47.83% (11/23) in normal skin,respectively.The expression of survivin was positively correlated with the high expression of PCNA in CA.The expression of survivin and PCNA was higher in HPV6/11 positive lesions than in HPV6/11 negative lesions. Conclusion HPV infection may up-regulate the expressions of survivin and PCNA,inhibit the apoptosis and promote the proliferation of keratinocytes.

Key words: Condylomata acuminata, Papillomavirus,human, Proliferating cell nuclear antigen, Survivin