中华皮肤科杂志 ›› 1988, Vol. 21 ›› Issue (4): 197-199.

• 论著 • 上一篇    下一篇

人表皮细胞单克隆抗体与天疱疮抗体的比较

翁孟武1, Safai. B2   

  1. 1. 上海医科大学皮肤病研究所;
    2. 美国Sloan-Kettering癌症研究中心
  • 收稿日期:1987-04-10 修回日期:1987-06-16 出版日期:1988-08-15 发布日期:1988-08-15

Distiction between epidermal monoclonal antibodies and pemphigus vulgaris autoantibodies

Weng Meng-wu1, Safai B.2   

  1. Institute of Dermatology, Shanghai Medical University
  • Received:1987-04-10 Revised:1987-06-16 Online:1988-08-15 Published:1988-08-15

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摘要： 抗人表皮细胞单抗H14和H19,用人皮肤作IIF检查,其荧光模式相同于天疤疮抗体所见.为此,对它们进行比较研究,结果如下:1.免疫荧光相互阻滞试验发现不能相互阻抑.2.抗原经不同溶液、温度和酶处理后,它们对荧光染色的反应各不相同.3. SDS-PAGE显示它们各沉淀不同分子量的抗原.上述结果提示它们是抗人表皮细胞膜上不同抗原成分的抗体.因此对表皮细胞示相同荧光染色,不一定代表是相同的抗体.H19染色复层鳞状上皮和肠道、肾小管上皮细胞,不着染其它组织,此单抗在肿瘤病理学上的作用尚需进一步研究.

Abstract: Two monoclonal antibodies (mAb), H14 and H19,were selected for detailed study since they were shown to give similar staining pattern for human epidermal cells to pemphigus vulgaris serum (Pab) by IIF. However, the antigen(s) recognized by mAbs and by Pab are probably not the same as suggested by the following: (1) No reciprocal blocking in immunofluorescence between them. (2) The binding of mAbs and Pab to human epidermal cells were affected differently by various conditions and agents. (3) H14 and H19 were shown to precipitate from surface-labeled human epidermal cells several proteins which are clearly distinguishable by SDS-PAGE to those precipitated by Pab. H19 stained all stratified squamous epithelia and most epithelial cells, but not other human tissues. These newly identified cell-surface antigens by H19 may be used as markers for the identification of human epidermal tumors.